DissolvIt ®
DissolvIt ® enables in vitro simulation of dissolution and absorption of respirable particles in the lung epithelium.
In the patented DissolvIt ® in vitro module: dry powder particles are deposited on a glass surface by PreciseInhale®. The particles are then brought into contact with a simulated lung/airway mucus and the dissolution is studied from the ‘luminal’ side through optical microscopy and from the ‘vascular’ side by chemical analysis of a flow-past perfusion medium.
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DissolvIt® – Technical Specifications
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The system consists of a dissolution chamber, a precision-controlled peristaltic pump and an inverted microscope with a high resolution camera. The dissolution chamber is perfused in single-pass mode after which the blood simulant is collected in a fraction collector. To further mimic the conditions in the lung, this system is heated to 37C.
Schematic overview of the DissolvIt® system. Particles pre-deposited on glass cover slip are sandwiched with a mucus simulant in the dissolution chamber. Dissolved particles absorbed by the single-pass perfusate are sampled by the fraction collector at selected timepoints. The dissolution process is monitored through the inverted microscope.
Key benefits and features:
- Ranks substances according to solubility
- Monitors absorption as well as dissolution
- Identifies successful, effective Drug Candidates early on
- Delivers exceptionally detailed data incl. Cmax and tmax curves
- Optical microscopy shows real-time dissolution of particles
- Customizable, programmable Fraction Collector for flexible sampling